Saturday 15 January 2005

High-toughness Spider Silk Fibers Spun from Soluble Recombinant Silk Produced in Mammalian Cells


Book Chapter
Book Title: Biopolymers Online

Polyamides and Complex Proteinaceous Materials
Authors:
  1. Dr. Costas N. Karatzas3
  2. Nathalie Chretien4
  3. François Duguay5
  4. Annie Bellemare6
  5. Dr. Jiang Feng Zhou7
  6. Dr. Andrew Rodenhiser8,
  7. Dr. Shafiul A. Islam9
  8. Carl Turcotte10
  9. Dr. Yue Huang11
  10. Dr. Anthoula Lazaris12
Published Online: 15 JAN 2005
DOI: 10.1002/3527600035.bpol8005
Jan 15, 2005  publication descriptionWiley Online Library
Publication Description:
Abstract
Introduction
Historical Outline
Production of rc-Spider Silk Proteins
Over-expression of Soluble rc-Spider silk Proteins in Mammalian Cells
Secretion of rc-Dragline Spider Silk proteins
Large-scale Production of rc-Spider Silk Proteins
Purification and Characterization of rc-Spider Silk Proteins
Production of rc-Spider Silk Proteins in the Milk of Transgenic Animals
Generation of Transgenic Mice for the Spider Dragline Silk Genes
Mammary Gland Production of Spider Dragline Silk Proteins
Recombinant Spider Silk-based Fiber Spinning
Spin Dope Preparation, Fiber Spinning and Characterization
Fiber Post-Spinning Treatment
Perspective on the Formation of Silk Fibers and the Spinning Process
Applications of rc-Spider Silk Fibers, and Prospects
Patents
Chronological Summary of Patents
Conclusions
Acknowledgments
Keywords:
spider silk; genetic engineering; recombinant silk protein; fiber spinning;expression; mammalian cells; transgenic mice; spin dope; fibers
More Info:
http://onlinelibrary.wiley.com/doi/10.1002/3527600035.bpol8005/full
http://onlinelibrary.wiley.com/doi/10.1002/3527600035.bpol8005/abstract